gibson assembly reactions (New England Biolabs)

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New England Biolabs gibson assembly reactions
Gibson Assembly Reactions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+reactions/Gibson+Assembly+Master+Mix/pmc12857616-190-0-9
Average 99 stars, based on 6279 article reviews

gibson assembly reactions - by Bioz Stars, 2026-09

99/100 stars

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Cloning:

Article Title: Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensor.
Article Snippet: .. Gibson assembly reactions were performed at 50 ◦ C for 1 h using the NEBuilder HiFi DNA assembly cloning kit (NEB E5520S), combining DNA fragments with overlapping sequences at equal molar ratios with the digested backbone sequence. .. The reaction product was transformed in NEB 5α competent E. coli (NEB C2987) and isolated using QIAprep Spin Miniprep Kit (Qiagen 27104).

Article Title: Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensor
Article Snippet: .. Gibson assembly reactions were performed at 50°C for 1 h using the NEBuilder HiFi DNA assembly cloning kit (NEB E5520S), combining DNA fragments with overlapping sequences at equal molar ratios with the digested backbone sequence. .. The reaction product was transformed in NEB 5α competent E. coli (NEB C2987) and isolated using QIAprep Spin Miniprep Kit (Qiagen 27104).

Sequencing:

Polymerase Chain Reaction:

Article Title: An optogenetics-compatible red fluorescent calcium indicator with negligible blue light photoactivation
Article Snippet: Fluorescence emission was passed through a Longpass Dichroic Beamsplitter (Chroma, T565lpxr) followed by an emission filter (Chroma, ET595/50m) and collected onto a GaAsP detector (Hamamatsu H10770) connected to a Becker & Hickl SPC-150 time-correlated single-photon counting (TCSPC) module. .. All PCR reactions were performed using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), and Gibson Assembly reactions were performed using NEBuilder HiFi DNA Assembly (NEB). .. All plasmids were sequence verified with nanopore sequencing (Plasmidsaurus).

other:

Article Title: RNA G-quadruplexes mediate cooperativity in HNRNPH binding and splicing regulation
Article Snippet: The PCR product as well as the donor vector pcDNA 3.1 (+) were digested with the Hind III-HF and Xba I restriction endonucleases (New England Biolabs), 20 units (U) each, and afterward again gel-purified.

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